等效电流极模型的脑电分析以及AD诊断中的应用

80年代以来，基于脑电图进行无创性病灶定位的研究，特别是基于等效电流极子定位法，得到了相当的发展并已经投入到正式的临床应用之中，因此等效电流极子模型在脑电图分析中逐步得到了广泛地应用。本文介绍并分析了等效电流极子模型在脑电分析以及阿尔滋海默病症（Alzheimer‘s Disease，简称AD）诊断中的应用状况。     

软鳞跳小蜂属一新种记述（膜翅目：跳中蜂科）

本文记述中国软鳞跳小蜂属１新种：壶蚧软鳞跳小蜂Ｌａｋｓｈａｐｈａｇｕｓ ｃｅｒｏｃｏｃｃｉ Ｘｕ ｅｔ Ｈｅ，ｓｐ．ｎｏｖ．。模式标本保存在浙江农业大学生物防治研究室。     

细菌的磷酸化作用

通常认为，在蛋白质上加入磷酸基是真核生物细胞主要的翻译后修饰调节。但是，愈来愈多的证据表明，这种修饰作用也存在于原核细胞，而且也有其功能，在MCP的这篇文章中，作者应用准确度高的质谱分析，结合磷酸多肽富集技术，     

可以用微波炉煎中药吗？

随着微波炉在家庭的普及，其用途越来越广。有些病人抓好药后，为了省事常常用微波炉煎中药，其实煎中药最好不要用微波炉。     

ω-3 多不饱和脂肪酸对母婴健康结局影响的研究进展

综述了国内外研究中ω-3 PUFAs补充与妊娠期间母婴健康结局的关系以及目前推荐摄入的国家的现状和推荐后所带来的健康效益，为我国妇女在妊娠期间是否需要补充剂ω-3 PUFAs以及其推荐摄入量提供科学依据。     

西藏报春花属(报春花科)一新变种

凤翔报春①新变种Ｐ．ｓｉｎｏｐｌａｎｔａｇｉｎｅａＢａｌｆ．ｆ．ｖａｒ．ｆｅｎｇｘｉａｎｇｉａｎａＷ．Ｌ．Ｚｈｅｎｇ，ｖａｒ．ｎｏｖ．Ａｖａｒ．ｓｉｎｏｐｌａｎｔａｇｉｎｅａｒｅｃｉｄｉｔｆｏｌｉｓｍａｊｏｒｉｂｕｓ，１２～１９ｃｍｌｏｎｇｉｓ，１?..     

细菌RNA分离试剂盒

Bioscience Technology 2003年7月31页报道：美国Amersham Biosciences公司最近推出GenomiPhi^TM DNA扩增试剂盒。利用这种试剂盒可从有限的生物材料制备高质量的DNA。这是一种简单的等温扩增技术，它不需要使用热循环器。原材料可以是来自全血、颊面拭子、细胞印迹纸、     

Immunological Properties of Recombinant Mycobacterium bovis Bacillus Calmette-Guérin Strain Expressing Fusion Protein IL-2-ESAT-6

The live vaccine Mycobacterium bovis bacillus Calmette-Guérin (BCG) provides variableefficacy against adult pulmonary tuberculosis (TB).Recombinant BCG,expressing either immunodominantantigens or Th1 cytokines,is a promising strategy for developing a new TB vaccine.However,not much isknown about whether the introduction of cytokine and specific antigen genes concurrently into the BCGstrain could improve the immunogenicity of BCG.In this study,a recombinant BCG strain (rBCG) expressingthe fusion protein human interleukin (IL)-2 and ESAT-6 (early secreted antigenic target-6 kDa) antigen ofMycobacterium tuberculosis was constructed.Six weeks after BALB/c mice (H-2~d) were immunizedwith 10~6 colony forming units (CFUs) BCG or rBCG,splenocyte proliferation was determined with MTT[3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide] assay,IL-4 and interferon (IFN)-γproducedby splenocytes were tested by enzyme linked immunosorbent assay (ELISA,) and the cytotoxicity ofsplenocytes from immunized mice to P815 cells (H-2~d) expressing ESAT-6 protein was measured usingCytoTox 96 Non-Radioactive Cytotoxicity Assay.Compared with native BCG-vaccinated mice,rBCG inducedstronger Th1 responses that were confirmed by high lymphoproliferative responses and IFN-γproductionto culture filtrate protein (CFP) or ESAT-6 protein.Moreover,rBCG induced significant enhanced CTLresponses against P815-ESAT-6 cells.Results from rBCG-immunized mice demonstrated that introducingthe il-2 and esat-6 genes into BCG could enhance Th1 type immune responses to ESAT-6.Further investigationis needed by introducing other Th1 cytokines and antigens into BCG to optimize the protective efficacyagainst TB.